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All cell lines except hTERT RPE-1 cells were cultured in DMEM (4.5 g l 1 glucose, Thermo Fisher Scientific) supplemented with 10% (v/v) FBS (Thermo Fisher Scientific), 100 U ml 1 penicillin (Thermo Fisher Scientific) and 100 g ml 1 streptomycin (Thermo Fisher Scientific)

doi: 10.2337/db10-0193 74 DunsheeDRBainbridgeTWKljavinNMZavala-SolorioJSchroederACChanRet al

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Many experienced users report optimal outcomes in the 0.25mg to 0.5mg range, with diminishing returns above that level

High-Efficiency Protection of Linear DNA in Cell-Free Extracts from Escherichia Coli and Vibrio Natriegens

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