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Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose

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This growing problem is largely attributed to the overuse of antibiotics in human and veterinary fields, which has become a critical global public health concern (Khalifa et al., 2021a

Our results showed that erastin negated the therapeutic effects of VNS, further confirming that VNS mitigates ferroptosis and exerts cardioprotective effects through the activation of the SLC7A11/GPX4 axis

Innovations continue toward fully synthetic organoid culture media, eliminating animal derived components to improve reproducibility and accelerate therapeutic adoption