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Muscle ammonia metabolism occurs via glutamate dehydrogenase (GDH) catalyzed ammonia-induced cataplerosis of a-ketoglutarate (aKG) to form glutamate

72 However, the principal regulatory enzyme responsible for lactylation is interchangeable with other PTMs, such as acetylation, leading to a lack of specificity

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This can be confirmed in the toxicity experiments of acute administration of high doses of LS-102, even when LS-102 is administered at a single-dose of up to 5,000 mg/kg body weight, no adverse reactions or even death are observed in mice (215)

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