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Effects of oral contraceptives and hormone replacement therapy on markers of cobalamin status

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Standard laboratory handling involves lyophilized 20 C storage, reconstitution in sterile or bacteriostatic water, and aliquoting to limit freeze-thaw cycles

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The use of 5AM-1MQ outside of this defined research context poses unacceptable, unquantified risks to human health

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Within MSFraggers, precursor mass tolerance was set to +/-20ppm, fragment mass tolerance was set to +/-20ppm, tryptic cleavage specificity was applied, as well as fixed methionine oxidation and fixed N-terminal acetylation

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