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While the identified pathology of -syn in DLB and PD spread from the brainstem to the limbic area and the neocortex [12, 13], -syn in AD with amygdala-predominant Lewy bodies (AD/ALB) may show a spread to lower regions from the upper neuraxis [14]

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Sections were washed in PBS-T and visualized by goat anti-mouse IgG (H + L) Alexa Fluor 488 (1:1,000, A32723, polyclonal, RRID AB_2633275, Thermo Fisher Scientific) or DyLight 488 (1:1,000, 35503, polyclonal, RRID AB_844397, Thermo Fisher Scientific) and DyLight 550 (1:1,000, SA5-10173, polyclonal, RRID AB_2556753, Thermo Fisher Scientific) secondary antibodies

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Hsieh from The University of Texas Southwestern Medical Center for tissue slide preparation