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After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)

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For HSC analysis, bone-marrow cells were obtained by crushing the long bones with staining media (calcium- and magnesium-free PBS with 2% FBS) using mortars and pestles