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For each experiment, a new vial of cryopreserved cells was thawed and utilized for both control and treated cells to ensure biological consistency while minimizing batch effects

Researchers observed that aortas from patients and animal models exhibit increased cellular ROS levels, oxidative DNA damage, and VSMC apoptosis

These exclusions ensured accurate determinations of baseline parameters

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146 Osteosarcoma cell lines and tissues show low expression of miR-144-3p and high expression of its target gene ZIB1, and miR-144-3p level is negatively correlated with the invasion and migration ability of osteosarcoma cells