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Briefly, sgRNA-expressing mCherry + cells were sorted as follows: GFP screen (brightest 30% GFP + and dimmest 30% GFP + ), BFP screens (brightest 30% BFP + and dimmest 30% BFP + ) and GFP:BFP screens (brightest 30% GFP:BFP + ratio and dimmest 30% GFP:BFP + ratio)

Discussion Earlier studies showed that IGF-1 receptors are expressed in the retina and outer and inner segments of the photoreceptors [31, 32]

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Visualizing data through charts and graphs can make trends more apparent, aiding in the interpretation of results and the refinement of future research protocols