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Neurological Function Score (Modified Longa System) Neurological deficits were evaluated 1 hour after ET-1 injection using the modified Longa scoring system: 0: No observable neurological deficit (excluded from analysis) 1: Failure to fully extend the left forepaw 2: Consistent circling to the left 3: Falling or leaning to the left side when ambulating 4: No spontaneous movement (severe infarction) Animals scoring between 1 and 3 were considered to have moderate ischemic deficits and were included in further analyses

Considerations Slower onset: Because the peptide must traverse the nasal membranes and then enter general circulation (and eventually reach brain/central nervous system targets), the onset tends to be more gradual

TB-500: The Logistics Coordinator that helps repair cells reach injury sites

Its effect on free-radical formation when topically applied to the skin by means of a cream has not been clearly established

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Then, the abundance of the natural 13 C-labeled metabolite was subtracted from the total 13 C-labeled metabolite in MCF7 cells cultured with 13 C 3 -lactate