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Some products require cold-chain storage, reconstitution, and self-injection using sterile technique

Protein extraction and western blotting For protein isolation, hDFs, iPSCs and MNPs were washed twice with ice-cold 1X phosphate saline buffer (1X PBS) and lysed with an extraction buffer containing 50 mM HEPES (Thermo Fisher Scientific), 150 mM NaCl (Applichem), 1 mM EDTA (pH8) (Applichem), 1 mM EGTA(pH8), 10% glycerol (Romil Pure Chemistry), and 1% Triton-X-100 (Applichem) complemented with protease (Sigma-Aldrich) and phosphatase inhibitors (PhosSTOP, Roche), according to the manufacturers protocol

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An incision was made on one side of the tumor, and the skin was separated from the tumor using surgical scissors