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Vitamin disturbance is common in high-risk populations for osteoporosis

Given that 2-AB has a similar structural formula to cysteine (Fig

10.3390/ijms26052367 32 DjordjeviV

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Ethanol-fixed cells were blocked, permeabilized, and incubated overnight at 4 C with a primary mouse monoclonal antibody to H2AX (Ser-139) (Upstate Biotechnology, Lake Placid, NY, USA) diluted 1:500, or mouse anti-active caspase 3 (BD, Pharmigen, San Diego, CA, USA) diluted 1:50 for 2 h at room temperature