l-tyrosine and acetyl l-carnitine together Acetyl-L-Carnitine Five unique ways L-Tyrosine can

Pole test This method was carried out as described in our previous work [65]

Chromatin fractionation Mitotic cells collected after 18 h of nocodazole treatment were lysed in fractionation buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM MgCl 2 , 10% glycerol, 0.2% NP-40) supplemented with 1 mM DTT, protease inhibitor cocktail (P8340, Sigma), 10 mM NaF, and 20 mM -glycerophosphate

DOI: 10.1084/jem.192.6.907

After washing of the membranes (1 5 min TBS, 2 5 min TBST, 3 5 min TBS), HRP-conjugated secondary antibodies diluted in M-TBST were applied to the blots

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