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That is a known class effect

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The cells were then plated onto 10-cm dishes coated with collagen I (Corning, 354236, 200 g per dish) for further culture

For instance, vitamin B12 has a half-life of 6-7 days, signifying that it takes roughly one week for half of the vitamin to be expelled from the body

Highlights: RT-AM is cell permeable and can generate RT upon intracellular esterase cleaveage Easily quantitate GSH levels in live cells using confocal microscopy/FACS Signals from blue and green channels allow for deduction of GSH concentration, given appropriate calibration curve For calibration curve, see: RealThiol (RT) Glutathione (GSH) Calibration Probe Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification

Using the same approach, we herein confirmed the successful fabrication of functionalized nerve protectors (NPs) made of porcine small intestine submucosal (SIS) extracellular matrix, whereby the decellularized wall matrix of NPs were repopulated with GiSCs as evidenced by the positive expression of S-100 in cells that have transmigrated into the wall matrix (data not shown)