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To measure kinetics rates and affinities, recombinant human FRa (Sino Biological cat#11241 -H08H) analyte sample is injected in duplicates at 5 concentrations (0.5, 1 , 2, 4, 8 nM) in series using single cycle kinetics strategy and at constant 70 pL/mL flow rate of running buffer (HBS-EP+, Cytiva cat# BR100669) with contact pulses of 300 sec

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aeruginosa , and Candida albicans

The advent of high-resolution mass spectrometry and sophisticated computational tools has revolutionized our ability to discover and characterize PTMs, revealing over 500 distinct modifications that play crucial roles in virtually every cellular process and human disease 1

PMID: 2858818