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For the dot blots, the protein concentration was normalized using BCA (Fisher Scientific, #PI23227) and 3g in 3L was spotted onto nitrocellulose membrane (Bio-Rad, #1620115)

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Upon activation, DCs transition their metabolic pathways towards anabolic metabolism, including glycolysis, fatty acid synthesis (FAS), and ROS production, to meet elevated energy and biosynthetic requirements[47]

Analyses of doxorubicin metabolism HCT116 cells (5 10 6 cells/10 cm dish) were cultured for 2 days, after which the cells were cultured in the presence of 0.3 mol l 1 doxorubicin overnight

Undergo a Thorough Medical Evaluation If you choose to proceed, we will schedule a comprehensive medical evaluation for your child