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5 Methods 5.1 Cell culture The THP-1 human leukemic monocytic cell line, obtained from the American Type Culture Collection, was cultured in RPMI-1640 medium (R0883-500ML, Sigma-Aldrich) supplemented with 10% heat-inactivated FCS, 2 mM L-glutamine, and 100 U/ml penicillin-streptomycin, and incubated at 37C in a 5% CO 2 atmosphere

This can lead to low self-esteem and a lack of confidence, harming a persons personal and professional life

doi:10.1006/bcmd.2002.0556

So dark leafy greens are high in magnesium

Most sessions take under 30 minutes

These limitations hinder the transition of ferroptosis-based strategies from invertebrate and rodent models to clinical settings