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Total RNA (500 ng) was then subjected to reverse transcription using a Superscript II reverse transcriptase kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions

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Cell viability assays Cells (3000 cells/well) for each condition were plated in 96-well plates and cultured in 100 l of the indicated medium containing 10% serum

ClinicalTrials.gov (2025) Related Dosing Protocols Written by Garret Grant Founder & Lead Researcher B.S

The selection of L-carnitine doses was based on the previous studies (Aboubakr et al., 2020), (Rajasekar et al., 2008)