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10.1038/oby.2007.285 331

The cells were collected, washed with 1 ml phosphate-buffered saline (PBS), and centrifuged for 5 min (1000 rpm) to remove the supernatant

Gronkiewicz KM, Giuliano EA, Kuroki K, Bunyak F, Sharma A, Teixeira LB, Hamm CW, Mohan RR

Subsequently, 2 joins the AAGAB: dimer to assemble into an AAGAB::2 trimer (the C form), where an :2 hemicomplex is formed and tethered to the CTD of AAGAB through a binding interface between and the CTD of AAGAB

The RRF value was calculated as the ratio of the absorbance read at = 570 to the absorbance value for = 700 nm

It increases even higher with use of corticosteroids (50%) [33] when compared with the general population