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Immunoblot analysis was performed on both total cell extracts (Input) and FLAG immunoprecipitates (IP:FLAG) using anti-HA antibodies to detect HA-Ub conjugates

In vitro studies have shown that elevating cytoplasmic Ca 2+ with the calcium ionophore A23187 led to a 2.9-fold upregulation of the nuclear-encoded NDUFA9, mitochondrial COX protein, and mitochondrial transcription factor TFAM [239, 240]

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[DOI] [PMC free article] [PubMed] [Google Scholar] 278.Pinckaers F.M.E., Rotee I.L.M., Nwosu C.V., Krolinski P., Smeets A.P.W., Glksz S., de Jong L., Vaessen T.S.J., Damen T., Uittenboogaard A., Schfer A.T., Menne-Lothmann C., Decoster J., van Winkel R., Collip D., Delespaul P., De Hert M., Derom C., Thiery E., Jacobs N., Wichers M., Rutten B.P.F., van Os J., Drukker M

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Many mass-market products use trace amounts of the peptide, just enough to list it on the label, in bases that arent designed to carry it past the outer skin layers