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The maintenance of energy generation, necessary for sperm motility is significantly impaired by defects in sperm mitochondrial activity, which may also be the underlying cause of asthenozoospermia (68)

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A 0.3ml (30-unit) insulin syringe with a 2931 gauge, 8mm needle is recommended for most doses in the 0.52mg range

This technique is a combination of two steps, namely emulsification and diffusion