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Transfections were performed in 24-well plates using 1-1.5 l of Trans IT-BrCa Transfection Reagent to deliver 0.5 g of DNA (2:1 and 3:1, reagent:DNA ratio)

Lu W, Eapen MS, Hardikar A, Chia C, Robertson I, Singhera GK, et al

Epithelial cells express NOX1 and DUOX2, which produce ROS into the extracellular space or gut lumen (121123), whereas phagocytes (neutrophils, eosinophils, macrophages) express NOX2 (phagocyte oxidase) that produces superoxide inside phagosomes or can leak it outside (124, 125)

67 Murine models Three main types of BCBM murine models exist: orthotopic models, intracarotid/intraventricular models and stereotactic intracranial injection models