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After all non-retained components are washed off the column, binding solute or together with ligand as solute-ligand complex are eluted by presence of mobile phase which was prepared in suitable pH, ionic strength and solvent composition for solute-ligand binding

Care Cancer 24 (7), 31053110

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The underlying principle involves the reaction of xanthine with xanthine oxidase, leading to the generation of superoxide radicals (O 2 )

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