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They were then incubated with primary antibodies at 4C overnight, washed three times with PBS for 5 min each and incubated with secondary fluorescent antibodies (Alexa 488/555/647, Invitrogen) at 1:500 dilution for 1 h at room temperature, washed 3 with PBS again and finally mounted with Vectashield (Vector, H1000) for imaging

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It was a similar sensation to watching an NBA player dribble absentmindedly during warm-upslazy movements occasionally punctuated by slick bursts of athleticism well beyond the realm of normal

Theyll talk to you about the necessary repairs, replacements or maintenance plans they recommend

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