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The cell line was cultured in basal bronchial epithelial cell basal medium (BEBM) (Lonza, Walkersville, MD) supplemented with 2 ml of bovine pituitary extract and 0.5 ml of: insulin, hydrocortisone, retinoic acid, transferrin, triiodothyronine, epinephrine, and human recombinant epidermal growth factor (Lonza, Walkersville, MD)

Leaves some moisture in the bowl if not dried

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One work published in the mid '50s, described it as "at best, an ungentlemanly and dirty habit," while for 1860 warns gentlemen that Cassell's Hand-book of Etiquette If you smoke or take snuff, you will find it difficult to observe that constant personal cleanliness so essential in a gentleman

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