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The amounts of total reduced and oxidized glutathione were quantified using a modified Tietze method by Adams (52)

These findings highlight a shift from a baseline inflammatory profile associated with ocular surface damage toward a post-treatment milieu in which cytokine expression parallels improvements in tear production and PROMs

European and African Division, Sixth Meeting 221, 1981

Mechanism of action and molecular effects of acetylation Mechanism of acetylation In acetylation modification, although the mass of the acetyl group is only 42 Da, the acetylation of the -amino group results in the neutralization of the inherent positive charge of lysine residues

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Both tissue and cellular RNA were extracted using the TRIzol reagent (15596026CN, Invitrogen) following the manufacturers protocol