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Briefly, the coding sequence (CDS) of HrGSTm1 was cloned into the pET-32a(+) vector (TaKaRa, Shiga, Japan) with Bam HI and Hin dIII restriction sites, then transformed into competent Escherichia coli (DE3) cells (AxyGen, Shanghai, China) and cultured at 37 C for 24 h

In tumor cells also, the expression level of ODC and the concentration of polyamines were found to be elevated [112]

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While useful, these tests can sometimes offer a narrow view of exposure, failing to quantify the level of mycotoxins present in the body, which are direct indicators of mold toxicity

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The aim is to elucidate novel therapeutic strategies and enhance understanding of the complex etiology of DE