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This process, often referred to as target validation , is a foundational step in early drug development

Slices were sectioned cross- and length-wise (400 ) to ensure penetrability of the drug, using a Mcllwain tissue chopper (Brinkmann, Gomshall, UK) and transferred to a gassed (95% 0 2 /5% CO 2 ) incubation chamber containing aCSF for 90 minutes at room temperature

In the earliest iterations, monolayer endothelial cells alone (BBB-on-chip) or with astrocytes and pericytes (NVU-on-chip) were cultured as in transwell systems (Jiang et al., 2021)

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Bloodbrain barrier permeability is induced in AOM mice via a mechanism involving the TGF 1-driven SMAD3-dependent upregulation of MMP9 expression and decrease of claudin-5 expression