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For perimenopause specifically, hormonal fluctuation drives many of the most disruptive symptoms estrogen can spike and crash erratically, which is why some patients feel worse in early perimenopause than in full menopause

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In the first protocol, growth factor-reduced matrigel was pipetted into prechilled 24-well plates (150 mL matrigel per well) and polymerized for 45 minutes at 37C

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