glutathione s transferases and drug resistance S-transferases P1-mediated interleukin-6 in tumor-associated macrophages augments drug-resistance in MCF-7 breast cancer Glutathione S-transferases in kidney and – Overexpression of Glutathione S-Transferases in

RNA isolation and RTqPCR Total RNAs from HeLa and HT-29 cells with or without the respective treatment were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturers instructions From the cDNA generated by the Iscript Reverse Transcription Supermix (Bio-Rad), RTqPCR was performed using Itaq Universal SYBR (Bio-Rad), with the primers listed in Supplementary Table 1 and the following conditions: initial polymerase activation step of 30 s at 95 C, followed by 35 amplification cycles of 5 s at 95 C and 30 s at 64 C

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133 ZhangN.HuY.DingC.ZengW.ShanW.FanH.et al (2017)

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