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THP-1 cells, a human acute monocytic leukemic cell line, were obtained from ATCC (TIB-202) and cultured in an incubator at 37 C, 5% CO 2 , and 95% humidity in RPMI-1640 medium containing 2 mM L-glutamine, 25 mM HEPES, 1% sodium pyruvate, 0.01% of 2-mercaptoethanol (2-ME), 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin

The protein we consume helps augment recovery and new muscle tissue from our resistance training,' says Yiannis Fleming, a sports performance coach and qualified nutritionist

10.1016/j.envint.2019.105029 51 KarriV.SchuhmacherM.KumarV

Immunohistochemistry analysis The colon samples were immediately fixed with 4% paraformaldehyde for 24 h upon separation, followed by paraffin embedding and cutting to a thickness of 4 m

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