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GSH was normally distributed, whereas GPx and the GSH:GSSG ratio were not normally distributed

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After 48 h, the luciferase activity was measured by Dual-Glo Luciferase Assay System (Promega, Madison, WI, USA) using microplate reader (GloMax DISCOVER, Promega, Madison, WI, USA)

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They found that the major metabolic abnormalities and increased levels of lipid hydroperoxides (the markers of oxidative stress) were observed in children of 410 years old but not in adults