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Nrf2-dependent upregulation of antioxidative enzymes: a novel pathway for proteasome inhibitor-mediated cardioprotection

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Blocking buffer composed of 0.3% v/v Triton-X, 0.05% Tween 20 (Sigma) and 3% bovine serum albumin (BSA) (Sigma), was added to fixed cell culture for 2 h gently rocking at room temperature