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2.6 16S rRNA gene sequencing After extracting the total DNA of the fecal sample, PCR amplification was performed and the product was purified

After lysis and release of the solubilized protein, the GST tagged protein is purified by affinity chromatography by adding 0.5ml glutathione resin to the clarified lysate

This is what we refer to as Adrenal Fatigue

Also, L Carnitine should not be taken by pregnant and lactating women, but this is a standard rule

Authors are thankful to Prof Amit Pathak for providing computational facility

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