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HeLa WT endogenous HA-GABARAPL2 cells were generated using CRISPR/Cas9 technology and kindly provided by the Behrends laboratory (LMU Mnchen, Germany)

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Treatment Overview & Aftercare Treatment duration: 30 minutes About Vitamin B12 Injections Vitamin B12 injections are a safe and effective way to replenish your B12 levels During the procedure Area cleansing at injection site Injection given into chosen area Before your appointment Avoid alcohol 24 hours before Increase water intake before treatment for optimum results Inform of any changes in medications or conditions Procedure details An injection is usually given into your upper arm, but your practitioner may choose a different site depending on your needs Duration of effect Our suggestion is to have one Vitamin B12 injection at weekly intervals, up to four doses (so four doses over four weeks)

'Leaving' groups During its reaction with DNA the dichloro groups of cisplatin are displaced or substituted but the two ammine groups remain intact, leading to a convention to refer to the groups within platinum cancer drugs that are displaced as 'leaving groups' (and those that remain as stable or carrier ligands)

The same recommendation applies for dapsone, a sulfone, which also does not need to be withheld

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