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Description

RNA purification and quantitative PCR Total RNA was isolated from cells and tissues using the RNeasy Mini Purification Kit (Qiagen) according to the manufacturers protocol

Solarski, M

Phlorizin ameliorates obesity-associated endotoxemia and insulin resistance in high-fat diet-fed mice by targeting the gut microbiota and intestinal barrier integrity

Materials and methods Induction of ischemic preconditioning and ischemia Male Wistar rats at the age of 3.54 months (having a mean weight of 380 26 g) were used