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Ltd., Shizuoka, Japan) or anti- F4/80 rat monoclonal antibody (abcam, Cambridge, UK) in the blocking solution at 4 C overnight

This calpain-mediated rearrangement of the erythrocyte cytoskeleton results in membrane blebbing [88, 89]

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Histological activity was A0/A1 in 74 cases (77%) and A2/3 in 22 (23%)

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reflects use history rather than current intoxication status Standard opioid immunoassay panels may produce false-negative results for hydrocodone specifically