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RNA extraction, cDNA synthesis and PCR amplification Total RNA was extracted from breast specimens by using the acid-phenol guanidium 64

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In that assay, a 1:1 mixture of mNEON-labeled H99 and the unlabeled sgf29 mutant was incubated with DMSO solvent or isoxazole 1 (32 g/mL) in RPMI/MOPS medium overnight at 37 C