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Sections were blocked with 2.5% normal horse serum (Vector Laboratories) for 1 h, followed by 24 h of incubation with the following rabbit primary antibodies: anti-S100 (1:100, Abcam ab41548), anti-MAOB (1:100, Abcam ab175136, Santacruz sc-515354), and anti-ABAT (1:100, Abcam ab108249)

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& Li, J

The same principle applies when using spray bottles

doi: 10.1016/j.heliyon.2024.e31413

This approach is the gold standard for treating conditions like spinal instability, deformities, and degenerative disc disease