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Staining: Ethanol fixed cells were washed twice with PBS at room temperature and incubated for 1 h at room temperature in the staining solution that contained: 50 l propidium iodide (1 mg/ml) (Sigma-Aldrich, St

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For measurements of oxidative damage we measured 3-nitrotyrosine (3NT), a measure of protein oxidation, and 3-chlorotyrosine (3CT), a measure of oxidative damage due to inflammation (myeloperoxidase activity)

Although low levels of reactive oxygen species (ROS) mediate critical physiological signaling pathways, accumulation of raised levels, if not cleared rapidly, can lead to major deleterious consequences affecting several organs, such as the heart, kidney, eye and brain, culminating in a variety of human disorders (Forman and Zhang 2021)