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Biochem Pharmacol 33:32413245

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The bacterial pellet was re-suspended in ice-cold 100 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer (pH 8.0) containing complete protease inhibitor cocktail (Sigma, United States) and phenylmethanesulfonyl fluoride (PMSF) then lysed by using zirconium beads on a bead beater homogenizer (MP Biomedicals FastPrep-24) for 1 min, five times for active cells and 10 times for dormant cells

The unbalance in pro- and anitioxidants may have increased susceptibility of brain PUFAs to oxidative damage and subsequently contributed to the deterioration of brain structure and cognitive impairment during the course of the disease [23, 24, 76, 8294]

Reported benefits included: Average systolic blood pressure reductions of 45 mm Hg in men Drops of 23 mm Hg in women Reductions of up to 11 mm Hg in overweight participants Some reductions were comparable to the effects of blood pressure medication

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