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Only the sites Cys15 and 102 were detected with a confidence precursor m/z

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Factors like light, temperature, oxygen supply, redox state, and metal ions significantly affect yield, leading to substantial batch-to-batch variation (Zhao et al., 2020

doi: 10.1016/j.reprotox.2018.09.008 20

Its performance characteristics include: Glutathione coupled to Sepharose High Performance, excellent for high resolution purification of GST-tagged proteins Easy one-step purification of GST-tagged proteins and other glutathione S-transferase or glutathionedependent proteins Prepacked GSTrap HP columns for simple operation with a syringe, pump or chromatographic system, such as KTAdesign These features make Glutathione Sepharose High Performance the ideal medium for high resolution purification of GST-tagged proteins, glutathione S-transferases and glutathione binding proteins