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DOI:10.1093/jnci/djt167

The biosynthesis of GSH involves formation of a peptide bond between glutamate and cysteine catalyzed by -glutamylcysteine synthetase (GshA) and subsequent formation of a peptide bond between Glu-Cys and glycine catalyzed by glutathione synthetase (GshB) [3]

C.GuermaziA.GuehringH.AydemirA.WaxS.Fleuranceau-MorelP.et al (2019)

Transformation : Introduce the plasmid into an appropriate expression system, such as Escherichia coli or yeast

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solubility, pH, and ionic strength should be validated per assay