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APC coordinates GSK3 phosphorylation of SETD8 to suppress colorectal cancer ScienceDirect Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns: STAR Protocols L Glutathione reduced, 70 18 8, G4251, Sigma Aldrich Calmodulin Sepharose 4B Cytiva 17 0529 01, pack of 10 mL Sigma Aldrich Glutathione Sepharose 4B, Cytiva, affinity resin, pack of 10 mL Glutathione Sepharose 4B GST tagged protein purification resin, 10mL
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