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Modest vasomotor dysfunction induced by low doses of C60 fullerenes in apolipoprotein E knockout mice with different degree of atherosclerosis

While this might suggest limited importance of the oxPPP in T reg cells, knockout of Pgd specifically in T reg cells (driven by Foxp3- Cre) causes severe T reg dysfunction in mice, which die because of autoimmunity between days 20 and 30 of life 119

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Similarly, in vitro binding assay with recombinant GSTO1 was performed using 1 M GSTO1 in reaction buffer (100 mM Tris (pH 8.0), 1.5 mM EDTA and 1 M DTT) incubated with compounds for 30 min at 37 C and with CMFDA (500 nM) for an additional 30 min

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Then, around 100 F1 L4 stage worms were transferred to the PA01-seeded plates supplemented with 5FU