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Immunoblotting Cells were washed with PBS at the end of treatments if applicable, and then lysed with an appropriate volume of 2X Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA, USA) with 5% -mercaptoethanol (Bio-Rad Laboratories)

Increases Collagen Production Collagen is an essential protein that makes up a significant portion of the human bodys tissues, including the skin, bones, tendons, and ligaments

IGF-1 is a peptide hormone that plays a crucial role in growth and development