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In this study, the N-terminal Myc-tagged Chac1 (Myc-Chac1) and Kozak-Chac1-Myc were stabilized by the HA-Ub (K63) co-transfection and MG132 treatment, whereas the HA-Ub (WT) co-transfection hardly affected the expression level of Myc-Chac1 (Fig