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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions

10.1111/j.1471-4159.2006.03907.x J Neurochem

While its pain-relieving effects are not as long-lasting as traditional painkillers like morphine, it appears to work quickly (within hours) to mitigate both inflammatory and nociceptive (injury-related) pain

Jun 13 2018;361:k2396

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The mechanisms are biologically plausible