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Without biotin vitamin B12 does not work

Preclinical Studies Early preclinical studies in rodent obesity models demonstrated that AOD9604 administration reduced body weight and fat mass without affecting food intake, indicating a direct metabolic effect rather than an appetite-suppressing mechanism

Preclinical and animal studies validate the clinical findings on AOD 9604 by demonstrating its clear impact on fat metabolism and 3-adrenergic receptor activity

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Summary: Yes, vitamin B12 is a water-soluble vitamin that dissolves in water and is excreted through urine, though uniquely it can be stored in the liver for several years

Without enough B12, serotonin production drops, leading to feelings of sadness or irritability, which can impact emotional well-being