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Description

Tasker and Mitchell-Heggs, 1976

Some of the preclinical assays described here were performed with the closely related precursor molecule, IMC-M113V RES , which differs from IMC-M113V by a single amino acid and demonstrates a similarly enhanced binding affinity to the cognate peptide HLA (pHLA)

Then, the reaction produces a fluorescent compound, dichlorofluorescein (DCF), which is determined at an excitation wavelength of 488 nm and an emission wavelength of 525 nm