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Sourris KC, Harcourt BE, Forbes JM (2009) A new perspective on therapeutic inhibition of advanced glycation in diabetic microvascular complications: common downstream endpoints achieved through disparate therapeutic approaches

The quantification of GSH was carried out by comparing the fluorescence produced by the samples versus that of a standard solutions curve with known concentrations of GSH.11 To measure GSSG concentration, 25L aliquots of saliva were incubated with 10L of a N-ethylmaleimide solution (NEM, 5mg/mL in distilled water) for 40min at room temperature

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Further, liver tissues were processed for frozen sectioning, hematoxylin and eosin (H&E) staining, and then micrometastases were counted by examination of H&E-stained sections under the microscope

Injury-induced pulmonary tuft cells are heterogenous, arise independent of key type 2 cytokines, and are dispensable for dysplastic repair